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96-multiwell boyden chambers  (Merck KGaA)

 
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    Merck KGaA 96-multiwell boyden chambers
    96 Multiwell Boyden Chambers, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/96-multiwell+boyden+chambers/96+multiwell+boyden+chambers/pm36906097-100-18-20
    Average 90 stars, based on 1 article reviews
    96-multiwell boyden chambers - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: 17-β Estradiol up-regulates energy metabolic pathways, cellular proliferation and tumor invasiveness in ER+ breast cancer spheroids
    Article Snippet: .. Afterwards, the cells were washed, re-suspended in free-serum DMEM medium, and placed in the upper compartment of 96-multiwell Boyden chambers (Merck Millipore, MA, USA) at a final concentration of 5×104 cells/well; the Boyden chamber lower compartment was filled with free-serum DMEM. .. Then, the Boyden chamber was incubated at 37°C and 95% air/5% CO 2 for 24 h. To detect invasive cells in the lower chamber compartment, cells were incubated with 60 nM calcein-AM for 1 h at 37°C and calcein-fluorescence was detected at 485 nm excitation and 520 nm emission by using a microplate reader (Nunclon, Roskilde, Denmark) ( ).

    Article Title: Energy Metabolism Drugs Block Triple Negative Breast Metastatic Cancer Cell Phenotype.
    Article Snippet: To establish alternative targeted therapies against triple negative (TN) breast cancer, (1) the energy metabolism and (2) the sensitivity of cell growth, migration and invasiveness towards metabolic, canonical and NSAID inhibitors was analyzed in MDA-MB-231 and MDA-MB468, two TN metastatic breast cancer cell lines, under both normoxia (21% O2) and hypoxia (0.1% O2).. For comparative purposes, analysis was also carried out in the less-metastatic breast MCF-7 cancer cells.. Under normoxia, oxidative phosphorylation (OxPhos) was significantly higher (2-times) in MDA-MB-468 than in MDA-MB-231 and MCF-7 whereas their glycolytic fluxes and OxPhos and glycolytic protein contents were all similar.

    Article Title: Acetate Promotes a Differential Energy Metabolic Response in Human HCT 116 and COLO 205 Colon Cancer Cells Impacting Cancer Cell Growth and Invasiveness
    Article Snippet: .. After 24 h, the cells were washed and resuspended again in fresh free-serum DMEM containing the respective SCCAs and placed in the upper compartment of 96-multiwell Boyden chambers (Merck Millipore; MA, USA) at a final concentration of 5 × 10 4 cells/well for another 24 h at 37°C. ..

    Article Title: 17-β Estradiol up-regulates energy metabolic pathways, cellular proliferation and tumor invasiveness in ER+ breast cancer spheroids.
    Article Snippet: .. Afterwards, the cells were washed, re-suspended in freeserum DMEM medium, and placed in the upper compartment of 96-multiwell Boyden chambers (Merck Millipore, MA, USA) at a final concentration of 5×104 cells/well; the Boyden chamber lower compartment was filled with free-serum DMEM. .. Then, the Boyden chamber was incubated at 37°C and 95% air/5% CO2 for 24 h. To detect invasive cells in the lower chamber compartment, cells were incubated with 60 nM calcein-AM for 1 h at 37°C and calcein-fluorescence was detected at 485 nm Frontiers in Oncology 04 excitation and 520 nm emission by using a microplate reader (Nunclon, Roskilde, Denmark) (18).

    Article Title: Estimation of energy pathway fluxes in cancer cells - Beyond the Warburg effect.
    Article Snippet: Glycolytic and respiratory fluxes were analyzed in cancer and non-cancer cells.. The steady-state fluxes in energy metabolism were used to estimate the contributions of aerobic glycolytic and oxidative phosphorylation (OxPhos) pathways to the cellular ATP supply.. The rate of lactate production corrected for the fraction generated by glutaminolysis is proposed as the appropriate way to estimate glycolytic flux.



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